Beilstein J. Org. Chem.2011,7, 813–823, doi:10.3762/bjoc.7.93
acetamides. The significant protecting effect of the hydroxyl radical and singlet oxygen scavengers to DNA cleavage was shown only with m-lysineconjugate. All three isomeric lysine conjugates inhibited human melanoma cell growth under photoactivation: The p-conjugate had the lowest CC50 (50% cell
cytotoxicity) value of 1.49 × 10−7 M.
Keywords: cancer cell proliferation assay; DNA alkylation; lysineconjugate; photocycloaddition; photo-DNA cleavage; plasmid relaxation assay; triplet excitation; Introduction
Triggering chemical processes with light offers numerous practical advantages. Not only does
potentially lead to a stronger pH-dependency on the photochemistry of the respective lysineconjugate, which is controlled by the protonation-gated intramolecular electron transfer from the α-amino group [25]. Interestingly, the meta-isomer has a noticeably longer singlet lifetime than the other two isomers